Maria Stefania Spagnuolo


Researcher (permanent position)

Email:; CNR PAGE

Phone: +39 0815966006

Fax: +39 0815965291

Address: ISPAAM-CNR, via Argine 1085, 80147 Napoli

Education and Professional Experience

2008-to date: Researcher (Permanent position)

December 2005-December 2007: Researcher (Temporary position)

March 2004-March 2005: Advanced Research Award, granted by POR Campania 2000/2006 (misura 3.13), at University of Naples Federico II, Department of General and Environmental Physiology.

October 2003-May 2005: Appointed as researcher in the project “Bioactive compounds in pasture species for phytotherapy and animal wellbeing” (ANFIT PROJECT), funded by Ministero delle Politiche Agricole e Forestali (DM 417757/26/06/01), at ISPAAM-CNR.

November 2000-October 2003: Ph.D. in Animal, Vegetal and Microbic Physiology at Department of General and Environmental Physiology, University of Naples Federico II

March 2000-November 2000: Fellowship granted by ISPAAM-CNR, attending the laboratory of Prof P.Abrescia, in the Department of General and Environmental Physiology, University of Naples Federico II.

March 1999-March 2000: Training post lauream at Department of General and Environmental Physiology, University of Naples Federico II.

February 1997-December 1998: Studentship in the laboratory of Prof. S. Filosa (Department of Evolutive and Comparative Biology, University of Naples Federico II).

December 2008: Degree with first class honours in Biological Sciences, University of Naples Federico II.


Molecular Physiology, Cell biology, Redox homeostasis, Oxidative stress, Inflammation, Animal welfare, Apolipoproteins, Haptoglobin, Cholesterol, Lactation, Environmental pollution.

Selected publications

  1. Spagnuolo M.S., Cigliano L., Balestrieri M, Porta A., Abrescia P. (2001). Synthesis of ascorbate and urate in the ovary of water buffalo. Free Radical Research, 35; 233-243.

  2. Balestrieri M., Spagnuolo M.S., Cigliano L., Storti G., Ferrara L., Abrescia P., Fedele E. (2002). Evaluation of oxidative damage in mozzarella cheese produced from bovine or water buffalo milk. Food Chemistry, 77; 293-299.

  3. Spagnuolo M.S., Cigliano L., Abrescia P. (2003). The Haptoglobin binding to Apolipoprotein AI: influence of Hemoglobin and Concanavalin A. Biological Chemistry, 384; 1593-1596.

  4. Spagnuolo M.S., Cigliano L., Sarubbi F., Polimeno F., Ferrara L., Bertoni G., Abrescia P. (2003). The accumulation of α-Tocopherol and Retinol in the milk of water buffalo is correlated with the plasma levels of Triiodothyronine. Biofactors, 19; 197-209.

  5. Spagnuolo M.S., Cigliano L., D’Andrea L.D., Pedone C., Abrescia P. (2005). Assignment of the binding site for haptoglobin on apolipoprotein A-I. Journal of Biological Chemistry, 280; 1193-1198.

  6. Spagnuolo M.S., Carlucci A., Cigliano L., Abrescia P. (2006). Nitric oxide stimulates the erythrocyte for ascorbate recycling. Nitric Oxide-Biology and Chemistry, 14; 272-277.

  7. Spagnuolo M.S., Vecchio D., De Rosa R., Polimeno F., Balestrieri A., Zicarelli G., Ferrara L., Campanile G. (2007). Effect of different housing conditions on several indices of blood redox status and on reproductive performance in buffalo cows. Italian Journal of Animal Science, 6 (Suppl. 2): 207-210.

  8. Cigliano L., D’Andrea L.D., Maresca B., Serino M., Carlucci A., Salvatore A., Spagnuolo M.S., Scigliuolo G., Pedone C., Abrescia P. (2008). Relevance of the amino acid conversions L144R (Zaragoza) and L159P (Zavalla) in the Apolipoprotein A-I binding site for Haptoglobin. Biological Chemistry, 389; 1421-1426.

  9. Cigliano L., Pugliese C.R., Spagnuolo M.S., Palumbo R., Abrescia P. (2009). Haptoglobin binds the anti-atherogenic protein apolipoprotein E – impairment of apolipoprotein E stimulation of both lecithin:cholesterol acyltransferase activity and cholesterol uptake by hepatocytes. FEBS Journal, 276; 6158-6171.

  10. Spagnuolo M.S., Cigliano L., Maresca B., Pugliese C.R., Abrescia P. (2011). Identification of plasma haptoglobin forms which loosely bind hemoglobin. Biological Chemistry, 392; 371-376.

  11. Spagnuolo M.S., Sarubbi F., Rossetti C., Grazioli G., Di Meo G.P., Iannuzzi L. (2011). Effect of dioxin exposure on several indices of blood redox status in lactating buffalo cows. Journal of Dairy Research, 78; 154-159.

  12. Genualdo V., Perucatti A., Iannuzzi A., Di Meo G.P., Spagnuolo S.M., Caputi-Jambrenghi A., Coletta A., Vonghia G., Iannuzzi L (2012). Chromosome fragility in river buffalo cows exposed to dioxins. Journal of Applied Genetics, 53; 221-226.

  13. Spagnuolo M.S, Cigliano L., Nebbia C., Rossetti C., Grazioli G., Iannuzzi L. (2012). Analysis of plasma indices of redox homeostasis in dairy cows reared in polluted areas of Piedmont (northern Italy). Science of The Total Environment, 433; 450-455.

  14. Spagnuolo M.S., Di Stasi R., De Rosa L., Maresca B., Cigliano L., D’Andrea L.D. (2013). Analysis of the haptoglobin binding region on the apolipoprotein A-I-derived P2a peptide. Journal of Peptide Science, 19; 220-226. DOI 10.1002/psc.2487.

  15. Cigliano L., Strazzullo M., Rossetti C., Grazioli G., Auriemma G., Sarubbi F., Iannuzzi C., Iannuzzi L., Spagnuolo M.S. (2014). Characterization of blood redox status of early and mid-late lactating dairy cows. Czech J. Anim. Sci. 59, 170-181.

  16. Spagnuolo M.S., Maresca B., Mollica M.P., Cavaliere G., Cefaliello C., Trinchese G., Esposito M.G., Scudiero R., Crispino M., Abrescia P., Cigliano L. (2014). Haptoglobin increases with age in rat hippocampus and modulates Apolipoprotein E mediated cholesterol trafficking in neuroblastoma cell lines. Front Cell Neurosci 8, 212. doi: 10.3389/fncel.2014.00212. eCollection 2014

  17. Spagnuolo M.S., Maresca B., La Marca V., Carrizzo A., Veronesi C., Cupidi C., Piccoli T., Maletta R.G., Bruni A.C., Abrescia P., Cigliano L. (2014). Haptoglobin interacts with Apolipoprotein E and beta-Amyloid and influences their crosstalk. ACS Chem Neurosci. 5, 837-847.

  18. La Marca V., Spagnuolo M.S., Cigliano L., Marasco D., Abrescia P. (2014). The enzyme lecithin-cholesterol acyltransferase esterifies cerebrosterol and limits the toxic effect of this oxysterol on SH-SY5Y cells. J Neurochem. 130, 97-108. doi: 10.1111/jnc.12713.

  19. Maresca B., Spagnuolo M.S., Cigliano L. (2015). Haptoglobin modulates beta-amyloid uptake by U-87 MG astrocyte cell line. J Mol Neurosci 56, 35-47. doi:10.1007/s12031-014-0465-6.

  20. Genualdo V., Perucatti A., Pauciullo A., Iannuzzi A., Incarnato D., Spagnuolo M.S., Solinas N., Bullitta S., Iannuzzi L. (2015). Analysis of chromosome damage by sister chromatid exchange (SCE) and redox homeostasis characterization on sheep flocks from Sardinian pasturelands. Sci Total Environ. 527-528, 393-400.

Istituto per il Sistema Produzione Animale in Ambiente Mediterraneo